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Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12

Hassan, M., Ashraf, Z., Abbas, Q., Raza, H

found that rotenone (a respiratory chain inhibitor that can increase mitochondrial ROS and total ROS) induced higher inflammasome activity of NLRP3 compared with lipopolysaccharide, indicating that ROS promoted the initiation step of NLRP3 assembly, and Nrf2 activation-induced NQO1 expression to clear ROS, thus inhibiting NLRP3 expression and subsequent caspase-1 cleavage ( Figure 5 ) (82)